1、ArcticExpress(DE3)RP大腸桿菌表達菌株信息:
貨號:MP1495
規(guī)格:300μl
儲存:-20℃
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抗性:慶大霉素Gen
培養(yǎng)基:LB
培養(yǎng)條件:37℃,有氧,LB
質粒轉化:42℃熱激
保存方式:20%甘油,-20℃
基本應用:用于蛋白表達
備注:
ArcticExpress Competent Cells are engineered to address the common bacterial gene expression hurdle of protein insolubility. These cells are derived from the high-performance Stratagene BL21-Gold competent cells, enabling efficient high-level expression of heterologous proteins in Escherichia coli.Low growth temperatures enhance protein folding and solubility Feature chaperonins Cpn60 and Cpn10 from psychrophilic bacterium Oleispira Antarctica Correct for codon bias and enhance solubility at the same time
6、注意事項:
1、為了您的安全和健康,請穿實驗服并戴一次性手套操作。本產(chǎn)品僅可用于實驗室研究,不能用于動物,人體以及作為食品添加劑等用途。
2、使用甘油菌時可不完全融解,在甘油菌表面蘸取少量涂布固體瓊脂平板即可,也可完全融解后使用,但隨著凍融次數(shù)的增加,
菌株的活力會逐漸下降。