国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > 行業(yè)資訊 > K-12 MG1655大腸桿菌克隆菌株

K-12 MG1655大腸桿菌克隆菌株

 K-12 MG1655大腸桿菌克隆菌株

貨號(hào)                          名稱                          規(guī)格 儲(chǔ)存
BMZ066590   K-12 MG1655 Strain 100μl -20℃
 
明舟生物的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證,如存在質(zhì)量問(wèn)題,請(qǐng)?jiān)谑盏疆a(chǎn)品三個(gè)月內(nèi)通知我司。收到甘油菌后,請(qǐng)及時(shí)在固體平板上劃線培養(yǎng),挑取單菌落接種到液體培養(yǎng)基中,震蕩培養(yǎng)后使用并保存甘油菌。如需獲取菌株其他詳細(xì)信息請(qǐng)登錄我司官網(wǎng)聯(lián)系查詢。
 
基因型:
F- lambda- ilvG- rfb-50 rph-1(NC_000913)
 
基本信息:
抗性:無(wú)
培養(yǎng)基:LB
菌株類別:大腸桿菌
培養(yǎng)條件:37℃,有氧,LB
質(zhì)粒轉(zhuǎn)化:42℃熱激
保存方式:20%甘油,-20℃
基本應(yīng)用:用于基因克隆
 
菌株簡(jiǎn)介:
   K-12 MG1655是一株K系列的大腸桿菌,經(jīng)檢測(cè)無(wú)Amp和Kan抗性,可用LB培養(yǎng)基,37℃培養(yǎng),其代謝類型是異養(yǎng)兼性厭氧型,可用于代謝研究、基因編輯等實(shí)驗(yàn)。
The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994).
MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine).
 
注意事項(xiàng):
1、為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。本產(chǎn)品僅可用于實(shí)驗(yàn)室研究,不能用于動(dòng)物,人體以及作為食品添加劑等用途。
2、使用甘油菌時(shí)可不完全融解,在甘油菌表面蘸取少量涂布固體瓊脂平板即可,也可完全融解后使用,但隨著凍融次數(shù)的增加,菌株的活力會(huì)逐漸下降。