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Animals were immunized with purified integrin associated protein (which is physically and functionally associated with vitronectin receptors).

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with vitronectin receptors on human polymorphonuclear leukocytes (PMN, neutrophils) and blocks the receptor binding to the tripeptide Arg-Gly-Asp (RGD).

The antibody also inhibits RGD stimulated phagocytosis.

It precipitates a heterodimer of apparent molecular weights 140000 and 105000 daltons.

The antibody does not react with gpIIb/III or the LFA-1/Mac-1 family of cellular adhesion receptors.

Complete culture medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Temperature: 37°C 
Atmosphere: 5% CO₂

Brown EJ. Novel monoclonal antibodies. US Patent 5,057,604 dated Oct 15 1991

Lindberg FP, et al. Rh-related antigen CD47 is the signal-transducer integrin-associated protein. J. Biol. Chem. 269: 1567-1570, 1994. PubMed: 8294396

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.