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8B1B.1
8B1B.1
規(guī)格:
貨期:
編號(hào):B162400
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) 8B1B.1
商品貨號(hào) B162400
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody is specific for the structural protein VP7.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Cellular Products
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bluetongue virus (serotype 17).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the structural protein VP7.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 x 104 viable cells/mL.  Maintain cultures at a cell concentration between 5 x 104 and 5 x 106 cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor JA House
Deposited As mouse (B cell); mouse (myeloma)
References

Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076

House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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