| 產(chǎn)品名稱 |
GA-10 |
| 商品貨號(hào) |
B162484 |
| Organism |
Homo sapiens, human |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Burkitt's lymphoma |
| Age |
41 years |
| Gender |
male |
| Ethnicity |
Caucasian, White |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The reported karyoype was 46, XY, dup(1) (pter to q32::q21 to qter), t(8;14) (q24.1; q32), -10, +der(10)t(Y;10)(q12:q26), -21, +der (21) t (?; 21) (?; p11)., In addition a few cells lacked a second chromosome 21 and carried an additional der(21) t(?;21) (?;q22). The Vh segment in this cell line was Vh4-34 (Vh 4.21). |
| Derivation |
GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy). |
| Clinical Data |
41 years
Caucasian
male |
| Antigen Expression |
CD10 +, CD19 +, CD20 +, CD40 + (10%), CD45 +, CD77 + (90%), CD34 - |
| Genes Expressed |
immunoglobulin; kappa light chain |
| Cellular Products |
immunoglobulin; kappa light chain |
| Comments |
The cells express relatively high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.
This cell line is slightly more sensitive to doxorubicin and etoposide than HL-60 leukemia cells.
Flavopiridol, a potent inhibitor of cell cycle progression through G1 and G2, produces apoptosis of the GA-10 cells at relatively low concentrations of 20-30 nM.
GA-10 cells are negative for Epstein-Barr virus (EBV). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,20 |
| Population Doubling Time |
24 hrs |
| Name of Depositor |
AP Rapoport |
| Deposited As |
human |
| Year of Origin |
1996 |
| References |
Rapoport AP, et al. Flavopiridol induces apoptosis and caspase-3 activation of a newly characterized Burkitt's lymphoma cell line containing mutant p53 genes. Blood Cells Mol Dis 27(3):610-24, 2001. PubMed: 11482875
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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