| 產(chǎn)品名稱 |
G355-5 |
| 商品貨號(hào) |
B162691 |
| Organism |
Felis catus, cat |
| Tissue |
brain |
| Cell Type |
astrocyte |
| Product Format |
frozen |
| Morphology |
glial, astrocyte |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
embryo |
| Applications |
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory. The line is useful for generating stocks of various C type retroviruses. |
| Derivation |
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory. |
| Comments |
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory. The line is useful for generating stocks of various C type retroviruses. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
heat-inactivated fetal bovine serum to a final concentration of 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin, add fresh trypsin (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
KJ Dunn |
| Deposited As |
Felis catus |
| References |
Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093
Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413
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