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Fr8-77
Fr8-77
規(guī)格:
貨期:
編號:B162725
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Fr8-77
商品貨號 B162725
Designations Fr8-77
Species Homo sapiens, human
Depositors G Scherer
Vector
Construct size (kb): 4.5
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: DNA Segment, single copy [D21S82]
Alleles: A3, B2, A1, A2, B1, B3
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; HindIII--4.4; EcoRI/HindIII--3.0, 1.45; BglI--4.4; PvuII--4.4.
Insert can be labeled by transcription with SP6 polymerase on HindIII linearized plasmid.
Codominant segregation shown in 2 informative families (10 individuals).
The locus D21S82 is very closely linked to the locus SOD1 on chromosome 21.
No background at stringency of 0.2X SSC, 65C.
Enzyme(s) not detecting polymorphism: BglII, HindIII, MspI, PstI, RsaI and TaqI, in 5 unrelated Caucasians.
References

Xiao GH, et al. Anonymous DNA sequence from chromosome 21 showing a three allele insertion/deletion RFLP (HGM 9 provisional no. D21S82). Nucleic Acids Res. 15: 5499, 1987. PubMed: 2885809

Warren AC, et al. A genetic linkage map of 17 markers on human chromosome 21. Genomics 4: 579-591, 1989. PubMed: 2568330

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