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Usutu virus
Usutu virus
規(guī)格:
貨期:
編號:B162819
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Usutu virus
商品貨號 B162819
Classification Flaviviridae, Flavivirus
Agent Usutu virus
Strain ENT MP 1626
Applications Vector-borne disease research
Emerging infectious disease research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1892 images
Comments Arbovirus
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero E6 cells (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 7-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor B Russell, CDC
Source Isolated from a mosquito in the Zika forest, Entebbe area, Uganda, 1962
References

Williams MC, et al. The Isolation of West Nile Virus from Man and of Usutu Virus from the Bird-Biting Mosquito Mansonia aurites (Theobald) in the Entebbe Area of Uganda. Ann Trop Med Parasitol 58: 367-374, 1964. PubMed: 14212897

Ashraf U, et al. Usutu Virus: An Emerging Flavivirus in Europe. Viruses 7: 219-238, 2015. PubMed: 25606971

Tetro JA. Is Usutu Virus Ready for Prime Time? Microbes Infect 19: 380-381, 2017. PubMed: 28619684

Pecorari M, et al. First Human Case of Usutu Virus Neuroinvasive Infection, Italy, August-September 2009. Euro Surveill 14(50): 19446, 2009. PubMed: 20070936

Santini M, et al. First Cases of Human Usutu Virus Neuroinvasive Infection in Croatia, August-September 2013: Clinical and Laboratory Features. J Neurovirol 21: 92-97, 2015. PubMed: 25361698

Gaibani P, et al. Comparative Genomic and Phylogenetic Analysis of the Usutu Virus Isolate from a Human Patient Presenting with Neurological Symptoms. PLoS One 8: e64761, 2013. PubMed: 23741387

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