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Bovine respiratory syncytial virus
Bovine respiratory syncytial virus
規(guī)格:
貨期:
編號(hào):B162837
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Bovine respiratory syncytial virus
商品貨號(hào) B162837
Classification Pneumoviridae, Orthopneumovirus
Agent Bovine respiratory syncytial virus
Strain A 51908
Applications Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Images VR-1861 images
Comments ATCC® VR-1861™ was derived from ATCC® VR-794™ following treatment with Mycoplasma Removal Agent (MRA) to remove Mycoplasma contamination.
Effect on Host
CPE, Syncytia with cell sloughing/detachment
Recommended Host
BT cells (ATCC® CRL-1390™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.

Name of Depositor SB Mohanty
Source Trachea of calf with respiratory disease
References

Mohanty SB, et al. Experimentally induced respiratory syncytial viral infection in calves. Am. J. Vet. Res. 36: 417-419, 1975. PubMed: 164807

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胡經(jīng)理 13345964880 2438244627
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于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479