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Vaccinia virus
Vaccinia virus
規(guī)格:
貨期:
編號:B162999
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Vaccinia virus
商品貨號 B162999
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain vTF7-3 [Wr]
Applications
Expresses bacteriophage T[7] RNA polymerase.
Expresses bacteriophage T[7] RNA polymerase.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Constructed in vitro
Product Format frozen
Storage Conditions -70°C
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
Cells which have been infected with this construct can be transfected by plasmids which contain a gene targeted for expression inserted between a T7 promoter and T7 terminator.
Expresses bacteriophage T[7] RNA polymerase.
Recombinant virus constructed in vitro.
Expresses bacteriophage T[7] RNA polymerase.
Expression of the T7 RNA polymerase is directed by the P7.5 promoter which includes early and late regulatory signals.
Constructed by transfecting vaccinia virus-infected cells with pTF703 (ATCC 67202).
Effect on Host
Yes, in vitro effects: Cytopathic effects (plaque formation) on BS-C-1 and CV-1 monolayers
CPE, rounding, stringing, and cell destruction
Name of Depositor NIH, B Moss, NIH
U.S. Patent
U.S. Patent Number
Source
Constructed in vitro
References

Fuerst TR, et al. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83: 8122-8126, 1986. PubMed: 3095828

Zheng Z, et al. Mutations in the carboxyl-terminal hydrophobic sequence of human cytomegalovirus glycoprotein B alter transport and protein chaperone binding. J. Virol. 70: 8029-8040, 1996. PubMed: 8892927

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