| 產(chǎn)品名稱 |
Hepatitis A virus |
| 商品貨號(hào) |
B163152 |
| Classification |
Picornaviridae, Hepatovirus |
| Agent |
Hepatitis A virus |
| Strain |
PA21 |
| Applications |
Hepatitis A antigen can be detected in infected cells by IF and in infected cell culture supernate by ELISA.
This item has fungal contamination that can, with difficulty, be controlled with Amphotericin B. Enteric Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
PA21 is one of the better characterized primate HAV strains that differs significantly from the HM175-like isolates [Brown et al., J, Virol. 63: 4932-37, 1989].
Hepatitis A antigen can be detected in infected cells by IF and in infected cell culture supernate by ELISA.
This item has fungal contamination that can, with difficulty, be controlled with Amphotericin B. |
| Effect on Host |
No CPE, detect by IF, ELISA or PCR |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 2.5 ug/ml Amphotericin B Solution and 2% fetal bovine serum
|
| Recommended Host |
BSC-1 (ATCC® CCL-26™)
|
| Growth Conditions |
Temperature: 35°C
Incubation: 20 to 28 days at 35°C, a 5% CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent). |
| Name of Depositor |
LN Binn |
| Special Collection |
ATCC |
| Source |
From liver tissue of a naturally infected owl monkey in Panama |
| References |
Binn LN, et al. Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures. J. Clin. Microbiol. 20: 28-33, 1984. PubMed: 6086708
|
