| 產(chǎn)品名稱 | Equid herpesvirus 1 |
|---|---|
| 商品貨號 | B163297 |
| Classification | Herpesviridae, Varicellovirus |
| Deposited As | Equine herpesvirus type 1 |
| Agent | Equid herpesvirus 1 |
| Strain | 438/77 |
| Common Name | Equine abortion virus |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation | Aborted equine fetus |
| Product Format | frozen |
| Storage Conditions | -70°C or colder |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images |  |
| Effect on Host | CPE, cell rounding and sloughing |
| Recommended Host | E. Derm (NBL-6) (ATCC® CCL-57™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | MJ Studdert |
| U.S. Patent | |
| U.S. Patent Number | |
| Source | Aborted equine fetus |
| References | Studdert MJ, et al. Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science 214: 562-564, 1981. PubMed: 6270790 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
