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13C4
13C4
規(guī)格:
貨期:
編號:B163566
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 13C4
商品貨號 B163566
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with the beta subunit of shiga toxin and SLTI and neutralizes Shiga toxin activities from Shigella dysenteriae type I and Shigella flexneri and Shiga-like toxin activities from Escherichia coli.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified shiga-like toxin from E. coli H30.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against shiga toxin and shiga like toxin I (SLTI)
Cellular Products
immunoglobulin; monoclonal antibody; against shiga toxin and shiga like toxin I (SLTI)
Comments
Animals were immunized with purified shiga-like toxin from E. coli H30.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the beta subunit of shiga toxin and SLTI and neutralizes Shiga toxin activities from Shigella dysenteriae type I and Shigella flexneri and Shiga-like toxin activities from Escherichia coli.
Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhimurium.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing

    Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/ml.

    Medium Renewal: Two  times a week.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype IgG1; kappa light chain
    Name of Depositor AD O'Brien
    References

    Strockbine NA, et al. Characterization of monoclonal antibodies against shiga-like toxin from Escherichia coli. Infect. Immun. 50: 695-700, 1985. PubMed: 3905611

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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