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AC133.1
AC133.1
規(guī)格:
貨期:
編號(hào):B163904
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 AC133.1
商品貨號(hào) B163904
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain New Zealand Black (NZB) (B cell); BALB/c (myeloma)
Applications
The antibody reacts with a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation

Animals were immunized with purified CD34 positive human progenitor stem cells, which had been pre-incubated with phytohemagglutinin (PHA).

Lymph node cells were fused with Sp2/0 Ag14 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a subset of human CD34 antigens
Cellular Products
immunoglobulin; monoclonal antibody; against a subset of human CD34 antigens
Tumorigenic Yes
Comments

The subset of cells recognized by AC133 is CD34bright, and contains substantially all of the CFU-GM activity present in the CD34+ population.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Isotype IgG1 kappa , IgG1; kappa light chain
Name of Depositor Amcell Corporation
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Yin A, et al. Characterization of a human hematopoietic progenitor cell antigen. US Patent 5,843,633 dated Dec 1 1998

Yin AH, et al. Human hematopoietic stem and progenitor cell antigen. US Patent 6,455,678 dated Sep 24 2002

Uchida N, et al. Enriched central nervous system stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations. US Patent 6,468,794 dated Oct 22 2002

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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