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Aedes albopictus [ATC-15]
Aedes albopictus [ATC-15]
規(guī)格:
貨期:
編號:B163913
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Aedes albopictus [ATC-15]
商品貨號 B163913
Organism Aedes albopictus, mosquito, Asian tiger
Tissue larva
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age larva
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Karyotype Karyotype stable within diploid stemline number. The chromosomes have median centromeres. In a few cases, a chromosome with a submedian centromere was noted. Two (2) cells with chromosome breaks (3%) and 6 cells with secondary constrictions (10%).
Images
Derivation

The Aedes albopictus [ATC-15] cell line was established from a pool of several hundred freshly hatched larvae.

Virus Susceptibility Dengue virus type 1
Dengue virus type 2
Dengue virus type 3
Colorado tick fever virus
Dengue virus type 4
Japanese encephalitis virus , Japanese encephalitis virus
Human poliovirus 1
Human herpesvirus 1 , Herpes simplex virus 1
Vaccinia virus
Vesicular stomatitis virus
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove culture medium to a centrifuge tube.
  2. Add 2.0 to 3.0 ml of 0.25%Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
  5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 7 to 8 x 103 viable cells/cm2 is recommended. Maintain cultures at a cell density between 1 x 104 and 1 x 105 cells/cm2.
  6. Incubate cultures at 28°C in 95% air, 5% CO2
Subcultivation Ratio: 1:3 to 1:6 
Medium Renewal: 1 to 2 times per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Complete growth medium supplemented with 10% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 28°C; (Max. 28°C, Min. 26°C)
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time 26
Name of Depositor SM Buckley
Deposited As Aedes albopictus
References

Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967.

Singh KRP, Paul SD. Multiplication of arboviruses in cell lines from from Aedes albopictus and Aedes aegypti. Curr. Sci. 37: 65-67, 1968.

Singh KR. Propagation of arboviruses in Singh's Aedes cell lines. I. Growth of arboviruses in Aedes albopictus and A. aegypti cell lines. Curr. Top. Microbiol. Immunol. 55: 127-133, 1971. PubMed: 5142320

Mitsuhashi J, Maramorosch K. Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22: 435-460, 1964.

Buckley SM. Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses. Proc. Soc. Exp. Biol. Med. 131: 625-630, 1969. PubMed: 5787147

Yunker CE, Cory J. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631-632, 1969. PubMed: 5798247

tissue from freshly hatched larvae

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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