| 產(chǎn)品名稱(chēng) |
AF6-122.2.5 |
| 商品貨號(hào) |
B163917 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with cells bearing the b, e and o haplotypes at the Igh-C locus. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Igh-5.3 (IgD b,e) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Igh-5.3 (IgD b,e) |
| Comments |
Animals were immunized with spleen cells from C57BL/6 mice. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with cells bearing the b, e and o haplotypes at the Igh-C locus. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
AM Stall |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Stall AM, Loken MR. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J. Immunol. 132: 787-795, 1984. PubMed: 6690619
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