Applications |
Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines. Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators. AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors. |
Comments |
AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines. Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators. AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP). AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP. The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors. |
References |
Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973
Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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