国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > anti-SRp20 (7B4)
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
anti-SRp20 (7B4)
anti-SRp20 (7B4)
規(guī)格:
貨期:
編號(hào):B163949
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 anti-SRp20 (7B4)
商品貨號(hào) B163949
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain.
The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins.
The antibody is used to determine if a factor in RNA metabolism is SRp20.
The antibody is useful for immunoprecipitation, immunoblotting and immunocytochemistry assays
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with FOX-NY myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against SRp20 proteins (pre-mRNA splicing factors)
Cellular Products
immunoglobulin; monoclonal antibody; against SRp20 proteins (pre-mRNA splicing factors)
Comments
Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain.
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins.
SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins).
The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons.
The antibody is used to determine if a factor in RNA metabolism is SRp20.
The antibody is useful for immunoprecipitation, immunoblotting and immunocytochemistry assays
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.  Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal:  Every 2 to 3 days

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor KM Neugebauer, MB Roth
Deposited As mouse (B cell); mouse (myeloma)
References

Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479