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AV3
AV3
規(guī)格:
貨期:
編號:B163966
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 AV3
商品貨號 B163966
Organism Homo sapiens, human
Tissue HeLa contaminant
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Derivation
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
HeLa Markers Y
Genes Expressed
The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
keratin
Comments
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor FC Robbins
Deposited As Homo sapiens
References

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

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