| 產(chǎn)品名稱 |
B27M1 |
| 商品貨號(hào) |
B163978 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/08A2 myeloma cells. |
| Clinical Data |
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic |
| Comments |
Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7). Spleen cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for HLA B27 and for HLA B7 at appropriate dilution. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
- 0.05 mM 2-Mercaptoethanol
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
F Grumet |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Grumet FC, et al. Monoclonal anti-HLA-B27 antibody (B27M1): production and lack of detectable typing difference between patients with ankylosing spondylitis, Reiter's syndrome, and normal controls. Lancet 2: 174-176, 1981. PubMed: 6166821
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