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B8-24-3
B8-24-3
規(guī)格:
貨期:
編號(hào):B163988
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 B8-24-3
商品貨號(hào) B163988
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Lymphoma
Applications
The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-2 b
Cellular Products
immunoglobulin; monoclonal antibody; against H-2 b
Comments
Animals were immunized with EL4 lymphoma cells mixed with Bordetella pertussis bacteria.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes.
It does not react with the d, f, j, k, q, s, v, u, w1 and W15 haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Isotype IgG1; kappa light chain
Name of Depositor G Kohler
Deposited As mouse (B cell); mouse (myeloma)
References

Kohler G, et al. Characterization of a monoclonal anti-H-2Kb antibody. Immune Syst. 2: 202-208, 1981.

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