產(chǎn)品名稱 |
BB7.6 |
商品貨號 |
B164001 |
Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
Cell Type |
hybridoma: B lymphocyte |
Product Format |
frozen |
Morphology |
lymphoblast |
Culture Properties |
suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Applications |
The antibody reacts with HLA B7,B40. The antibody cross-reacts cells of various primates. Tested and found negative for ectromelia virus (mousepox). |
Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA B7,B40 |
Cellular Products |
immunoglobulin; monoclonal antibody; against HLA B7,B40 |
Comments |
Animals were immunized with papain solubilized HLA A2,B7 antigen. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with HLA B7,B40. The antibody cross-reacts cells of various primates. Tested and found negative for ectromelia virus (mousepox). |
Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below) with 10(-4) M hypoxanthine, 1.6 x 10(-5) M thymidine, 90%; fetal bovine serum, 10%. DME w/HEPES (10mM) 100 mL L-Glutamine (100x) 1 mL Solution I 1 mL Non-essential amino acids (100x) 1 mL NCTC 135 10 mL Fetal Bovine Serum 12 mL Solution I (100x) 1. 1320 mg oxalacetic acid (100 mM, MW 132). 2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg). Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to 100 mL with distilled water. Stir at 37°C until solution dissolves. Filter, aliquot and store frozen. |
Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
Isotype |
IgG1 |
Name of Depositor |
P Parham |
Deposited As |
mouse (B cell); mouse (myeloma) |
References |
Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474
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