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BC-1
BC-1
規(guī)格:
貨期:
編號(hào):B164007
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BC-1
商品貨號(hào) B164007
Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 CELLS CONTAIN HERPESVIRUS

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Age 46 years
Gender male
Applications

Transfection host.

It is also a practical source of KSHV DNA to be used as a positive control for screening tests.

Storage Conditions liquid nitrogen vapor phase
Derivation

BC-1 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.

The cells were derived from an acquired immunodeficiency syndrome (AIDS) related body cavity based lymphoma presenting as a primary lymphomatous effusion in the peritoneal cavity.

Antigen Expression
CD30 +; CD45 +; EMA + (epithelial membrane antigen)
Comments

The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).

KSHV is a recently identified and largely uncharacterized virus.

The BC-1 cell line allows in vitro culture of KSHV viral genomes.

Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 105 cells/mL and maintain between 5 x 105 and 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8
D16S539: 12,13
D5S818: 11,12
D7S820: 11
THO1: 9.3
TPOX: 8,9
vWA: 16,20
Population Doubling Time 48 to 72 hrs
Name of Depositor E Cesarman
Deposited As Homo sapiens
References

Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568

Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109

Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575

Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311

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