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BE(2)-M17
BE(2)-M17
規(guī)格:
貨期:
編號(hào):B164019
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) BE(2)-M17
商品貨號(hào) B164019
Organism Homo sapiens, human
Tissue brain; derived from metastatic site: bone marrow
Cell Type neuroblast
Product Format frozen
Morphology neuroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease neuroblastoma
Age 2 years
Gender male
Applications This cell line is a suitable transfection host.
Derivation
BE(2)-M17 is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a 2 year old male with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Clinical Data
Patient had repeated courses of chemotherapy and radiotherapy.
male
Comments
BE(2)-M17 cells have a reported saturation density of greater than 1 x 106 cells/cm2
The cells grow as multilayering, neuroblastic cells with occasionally long neuritic processes.
As cultures age, cells will aggregate and float.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium carefully. Add 1-2 mL trypsin (0.25%) - EDTA (0.53mM) solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh culture medium, triturate cells and dispense into new culture flasks.


Subcultivation ratio: 1:20 to 1:50 at 1 to 2 week intervals is recommended
Medium Renewal: Every 4 to 7 days. pH should be less than or equal to 7.3.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 18
Population Doubling Time 20 to 24 hrs
Name of Depositor JL Biedler
Deposited As Homo sapiens
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479