產(chǎn)品名稱 | BEAS-2B |
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商品貨號 | B164023 |
Organism | Homo sapiens, human |
Tissue | lung, bronchus |
Cell Type | epithelial virus transformed |
Product Format | frozen |
Morphology | epithelial |
Culture Properties | adherent |
Biosafety Level | 2 [Cells contain polyomavirus DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | normal |
Applications | This cell line is a suitable transfection host. The cells retain the ability to undergo squamous differentiation in response to serum, and can be used to screen chemical and biological agents for ability to induce or affect differentiation and/or carcinogenesis. |
Storage Conditions | liquid nitrogen vapor phase |
Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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Derivation | Epithelial cells were isolated from normal human bronchial epithelium obtained from autopsy of non-cancerous individuals. The cells were infected with an adenovirus 12-SV40 virus hybrid (Ad12SV40) and cloned. |
Clinical Data | Epithelial cells were isolated from normal human bronchial epithelium obtained from autopsy of healthy individuals. |
Tumorigenic | The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
Effects | Yes, the cells did form colonies in semisolid medium No, the cells were not tumorigenic in immunosuppressed mice |
Comments | The cells stain positively for keratins and SV40 T antigen.
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Complete Growth Medium | The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit.
Note: Do not filter complete medium.
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Subculturing |
These cells should be subcultured before reaching confluence since confluent cultures rapidly undergo squamous terminal differentiation. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Interval: Subcultured before reaching confluence.
Medium Renewal: Every 2 to 3 days
Flask Coating
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Cryopreservation | Freeze medium: Complete growth medium supplemented with 1% PVP and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
STR Profile | Amelogenin: XY CSF1PO: 9, 12 D13S317: 13 D16S539: 12 D5S818: 12,13 D7S820: 10, 13 THO1: 7, 9.3 TPOX: 6, 11 vWA: 17, 18 |
Name of Depositor | The United States of America |
U.S. Patent Number | |
References | Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 Lechner JF, LaVeck MA. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985. Sakamoto O, et al. Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. J. Clin. Invest. 99: 701-709, 1997. PubMed: 9045873 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
梅經(jīng)理 | 17280875617 | 1438578920 |
胡經(jīng)理 | 13345964880 | 2438244627 |
周經(jīng)理 | 17757487661 | 1296385441 |
于經(jīng)理 | 18067160830 | 2088210172 |
沈經(jīng)理 | 19548299266 | 2662369050 |
李經(jīng)理 | 13626845108 | 972239479 |