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BT-483
BT-483
規(guī)格:
貨期:
編號:B164051
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 BT-483
商品貨號 B164051
Organism Homo sapiens, human
Tissue mammary gland; breast
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease ductal carcinoma
Age 23 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 72; range = 46 to 130
Images
Derivation The BT-483 line was isolated by E.Y. Lasfargues and W.G. Coutinho from a papillary invasive ductal tumor obtained from a 23 year old, normal, menstruating, parous female patient with family history of breast cancer.
Clinical Data
23 years
Caucasian
female
Tumorigenic Yes
Effects
Yes, in Amsterdam/IMR rats
Yes, in nude mice
Yes, in soft agar
Comments
The cells were highly pleomorphic with prominent nuclei and nucleoli [PubMed ID: 212572]. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy [PubMed ID: 212572].
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.
Subculturing Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37°C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 5,9.3
TPOX: 8,11
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 2
PGM3, 2
Name of Depositor EY Lasfargues
Deposited As Homo sapiens
References

Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479