| 產(chǎn)品名稱 |
BTV10XSp2/0-Ag14-10D4.90 |
| 商品貨號 |
B164054 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma:lymphoblast B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Strain |
|
| Applications |
The antibody reacts with a viral protein associated with the replication BTV. It can be used in immunofluorescence assays to identify the U.S. serotypes of BTV, and differentiate between BTV and the closely related virus, epizootic hemorrhagic disease virus (EHDV). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Bluetongue virus (BTV, orbivirus) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Bluetongue virus (BTV, orbivirus) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with serotype 10 BTV. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a viral protein associated with the replication BTV. It can be used in immunofluorescence assays to identify the U.S. serotypes of BTV, and differentiate between BTV and the closely related virus, epizootic hemorrhagic disease virus (EHDV). The immunofluorescence pattern is characterized as abundant discrete granules and large and small spherical inclusions in the cytoplasm of infected cells. |
| Complete Growth Medium |
Culture medium: HB101, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a kappa |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Jochim MM, Jones SC. Monoclonal antibodies to bluetongue virus antigen. US Patent 4,873,189 dated Oct 10 1989
. . Proc. Am. Assoc. Vet. Lab. Diag. : 277-283, 1983.
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