国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > C127I
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
C127I
C127I
規(guī)格:
貨期:
編號(hào):B164069
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 C127I
商品貨號(hào) B164069
Organism Mus musculus, mouse
Tissue mammary gland
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender female
Strain RIII
Applications

Useful for visualization of sarcoma virus-induced foci and for an in vitro quantitative assay for bovine papilloma virus.

Host for transfection with plasmids containing bovine papilloma virus DNA.

C127I cells are suitable hosts for transformation with bovine papilloma virus DNA plasmids (ATCC 37110, ATCC 37111, ATCC 37112, ATCC 39475)

Storage Conditions liquid nitrogen vapor phase
Derivation
Non-transformed clonal line derived from a mammary tumor of an RIII mouse.
Comments Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium

Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; fetal bovine serum, 10%

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (RefBerthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc. Natl. Acad. Sci. USA 83: 2496–2500, 1986. PubMed: 3458212). The Phenol Red-free version of DMEM, is ATCC catalog No. 30-2601.

Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37°C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week
Name of Depositor PM Howley
Deposited As Mus musculus
References

Sarver N, et al. Bovine papilloma virus deoxyribonucleic acid: a novel eucaryotic cloning vector. Mol. Cell. Biol. 1: 486-496, 1981. PubMed: 6100967

Lowy DR, et al. Helper-independent tranformation by unintegrated Harvey sarcoma virus DNA. J. Virol. 26: 291-298, 1978. PubMed: 26810

Dvoretzky I, et al. A quantitative in Vitro focus assay for bovine papilloma virus. Virology 103: 369-375, 1980. PubMed: 6247821

Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479