| 產(chǎn)品名稱 |
C32 |
| 商品貨號(hào) |
B164084 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
melanoma, amelanotic |
| Age |
53 year old adult |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
C32 was derived from a human male with skin melanoma. |
| Clinical Data |
53 year old adult Caucasian male |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 11,12 D5S818: 12 D7S820: 9,11 THO1: 9.3 TPOX: 9 vWA: 14,19 |
| Name of Depositor |
TR Chen |
| Deposited As |
Homo sapiens |
| References |
Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716
Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368
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