| 產(chǎn)品名稱 | C6/LacZ |
|---|---|
| 商品貨號(hào) | B164098 |
| Organism | Rattus norvegicus, rat |
| Tissue | brain |
| Cell Type | glial cell |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | glioma |
| Strain | outbred |
| Applications | The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production. The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. This is one of few models that permit quantitative analysis of microscopic tumor in the brain. C6/lacZ, but not 9L/lacZ, shows infiltrative growth in the brain. The beta-gal expression is less stable than for 9L/lacZ, and the cells should be used soon after thawing or re-cloned. |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | The C6/lacZ cell line was developed in 1989 from the C6 cell line (N-nitrosomethylurea induced rat glial tumor). The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. |
| Genes Expressed | beta galactosidase (beta-gal) |
| Cellular Products | beta galactosidase (beta-gal) |
| Tumorigenic | Yes |
| Effects | Yes, forms tumors in the brains of CD Fischer 344 rats |
| Comments | The C6/lacZ cell line was developed in 1989 from the C6 cell line (N-nitrosomethylurea induced rat glial tumor). C6 cells were infected with the BAG replication deficient retroviral vector carrying the E. coli lacZ gene encoding beta-gal and the Tn5 neomycin gene, which confers resistance to G418. The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production. The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. This is one of few models that permit quantitative analysis of microscopic tumor in the brain. The tumor mimics important features of human brain tumor growth and spread. The growth pattern of 6C/lacZ complements that of 9L/lacZ (see ATCC CRL-2200). C6/lacZ, but not 9L/lacZ, shows infiltrative growth in the brain. The beta-gal expression is less stable than for 9L/lacZ, and the cells should be used soon after thawing or re-cloned. |
| Complete Growth Medium | Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor | LA Lampson |
| Deposited As | Rattus sp. |
| References | Lampson LA, et al. Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res. 53: 176-182, 1993. PubMed: 8416743 Lampson LA, et al. Disseminating tumor cells and their interactions with leukocytes visualized in the brain. Cancer Res. 52: 1018-1025, 1992. PubMed: 1737331 Lampson LA, et al. A new model permits study of the immune response to individual tumor cells within the brain. Neurology 40: 396-397, 1990. Dutta T, et al. Robust ability of IFN-gamma to upregulate class II MHC antigen expression in tumor bearing rat brains. J. Neuro-Oncol. 64: 31-44, 2003. PubMed: 12952284 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
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