| 產(chǎn)品名稱 |
CCD 841 CoTr |
| 商品貨號(hào) |
B164149 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Cell Type |
SV40 transformed |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
21 weeks gestation |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The karyology of this line changed dramatically from the parental, but there are reports in the literature of SV40 causing such cytogenetic instability. STR results have consistently shown that CRL-1807 is 100% related to CRL-1790. This is a pentaploid human cell line, probably of female origin. The modal chromosome number is 113 occurring in 14% of cells; cells with 114 chromosomes occurred also at a high rate (10%). The rate of polyploid cells was 3.4%. Most cells had 12-15 markers. Among these, t(1p18q), del(7)(q22q32), t(6p22q), and del(12)(q26.1) were common to most cells. There were 4-5 X chromosomes per cell. Modal copy number per cell for autosomes was 5; N4, N8, N10, N16, N19 and N22 had relatively wide ranges of number of copies per cell. Karyotypes are very heterogeneous. |
| Derivation |
This line was derived from the same tissue sample as CCD 841 CoN (see ATCC CRL-1790) by transformation with a temperature sensitive mutant of SV40. |
| Comments |
The cells exhibit the transformed phenotype at the permissive temperature (33°C) for the virus. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 33°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 33°C |
| Name of Depositor |
A Thompson |
| Deposited As |
Homo sapiens |
