| 產(chǎn)品名稱 |
CCD-112CoN |
| 商品貨號(hào) |
B164178 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
22 weeks gestation |
| Gender |
female |
| Karyotype |
46, XX; modal number = 46; polyploidy at 5.5%; inv(9)(p13q13) |
| Clinical Data |
female |
| Comments |
The line begins to senesce at population doubling level (PDL) = 76. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 9,13 D13S317: 12 D16S539: 10,11 D5S818: 12 D7S820: 8,9 THO1: 6,7 TPOX: 8 vWA: 16,18 |
| Population Doubling Capacity |
The line begins to senesce at population doubling level (PDL) = 76. |
| Name of Depositor |
ATCC |
| Deposited As |
Homo sapiens |
