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CCD-1139Sk

規(guī)格:

貨期:

編號:B164193

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱	CCD-1139Sk
商品貨號	B164193
Organism	Homo sapiens, human
Tissue	skin
Cell Type	fibroblast
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	62 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase
Clinical Data	male Caucasian 62 years
Comments	Cells senesce after approximately 52 population doublings.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: 1:2 to 1:6 is recommened Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseliquid nitrogen vapor phase
Culture Conditions	Temperature: 37.0°C
Name of Depositor	P.Wood, ATCC
Deposited As	human

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