產(chǎn)品名稱(chēng) |
CCD-11Lu |
商品貨號(hào) |
B164195 |
Organism |
Homo sapiens, human |
Cell Type |
fibroblast |
Product Format |
frozen |
Morphology |
fibroblast |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Age |
9 years |
Gender |
male |
Ethnicity |
Asian |
Applications |
The line was derived from normal tissue from patient who died following trauma to the head. |
Karyotype |
normal human male; diploid; stable |
Derivation |
The line was derived from normal tissue from patient who died following trauma to the head. |
Clinical Data |
The line was derived from normal tissue from patient who died following trauma to the head. male Asian 9 years |
Antigen Expression |
blood type O; Rh + |
Genes Expressed |
blood type O; Rh + |
Comments |
The line was derived from normal tissue from patient who died following trauma to the head. The cells are capable of at least 12 population doublings beyond the current freeze. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin for several minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. |
STR Profile |
Amelogenin: X,Y CSF1PO: 11 D13S317: 8,14 D16S539: 9,12 D5S818: 11,13 D7S820: 10,12 THO1: 9 TPOX: 8,11 vWA: 18,20 |
Name of Depositor |
GP Maxwell |
Deposited As |
Homo sapiens |