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CCD-19Lu
CCD-19Lu
規(guī)格:
貨期:
編號:B164199
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


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    產(chǎn)品名稱 CCD-19Lu
    商品貨號 B164199
    Organism Homo sapiens, human
    Tissue lung
    Cell Type fibroblast
    Product Format frozen
    Morphology fibroblast
    Culture Properties adherent
    Biosafety Level 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Disease normal
    Age 20 years
    Gender female
    Ethnicity Caucasian
    Storage Conditions liquid nitrogen vapor phase
    Karyotype normal female; diploid; stable
    Derivation
    The line was derived from normal tissue from patient who died of trauma to the head.
    Clinical Data
    20 years
    Caucasian
    female

    Comments
    The cells are capable of at least 8 or 9 population doublings beyond the current freeze.
    Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Subculturing

    Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

    Medium Renewal: Twice per week

    Cryopreservation
    Culture Conditions Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    STR Profile Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor
    Amelogenin: X
    CSF1PO: 10,12
    D13S317: 11,12
    D16S539: 11
    D5S818: 12
    D7S820: 9,11
    THO1: 9.3
    TPOX: 8
    vWA: 17,18
    Deposited As S Dilworth
    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479