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CCD-33Co
CCD-33Co
規(guī)格:
貨期:
編號(hào):B164205
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CCD-33Co
商品貨號(hào) B164205
Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 7 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This cell line has a trisomic N7 male karyotype, 47,XY,+7 in the majority of cells. However, cells with the normal 46,XY karyotype were also found. The modal chromosome number was 47, occurring in 62% of cells, and cells with 46 chromosome counts also occurred at a high rate at 32%. Apparently this is a mosaic population with the predominant trisomic N7 cells. The rate of polyploidy was 2.0%. Both X and Y chromosomes were present. No other consistent aberrations were detected in all analyzed cells.
Images
Clinical Data
7 years
Caucasian
male
Comments
Cells have the capacity to proliferate to a maximum of 29 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 11,13
THO1: 6,9.3
TPOX: 9,11
vWA: 15,16
Name of Depositor S Dilworth
Deposited As Homo sapiens
Year of Origin June 8, 1979
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479