產(chǎn)品名稱 |
CCD-34Lu |
商品貨號(hào) |
B164207 |
Organism |
Homo sapiens, human |
Tissue |
lung |
Cell Type |
fibroblast |
Product Format |
frozen |
Morphology |
fibroblast |
Culture Properties |
adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
normal |
Age |
2.5 weeks |
Gender |
female |
Ethnicity |
Black |
Applications |
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497. |
Derivation |
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497. |
Clinical Data |
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497. female Black 2.5 weeks |
Comments |
The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. |
Culture Conditions |
Temperature: 37.0°C |
STR Profile |
Amelogenin: X CSF1PO: 11,13 D13S317: 9,13 D16S539: 8,13 D5S818: 12 D7S820: 10 THO1: 7,9 TPOX: 11 vWA: 15,16 |
Name of Depositor |
GP Maxwell |
Deposited As |
Homo sapiens |