產(chǎn)品名稱 |
CCRF-SB [CCRF SB] |
商品貨號 |
B164219 |
Organism |
Homo sapiens, human |
Tissue |
peripheral blood |
Cell Type |
B lymphoblast |
Product Format |
frozen |
Morphology |
lymphoblast |
Culture Properties |
suspension |
Biosafety Level |
2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
acute lymphoblastic leukemia |
Age |
11.5 years |
Gender |
Male |
Ethnicity |
Caucasian |
Storage Conditions |
liquid nitrogen vapor phase |
Images |
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Derivation |
Derived from a buffy coat preparation from a patient with acute lymphoblastic leukemia.
A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.
|
Clinical Data |
11.5 years
Caucasian
male |
Antigen Expression |
HLA A1, A2, B12, B17, Cw2 |
Comments |
The cells are EBNA positive |
Complete Growth Medium |
RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
|
Subculturing |
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 105 viable cells/mL.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days |
Cryopreservation |
culture medium 95%; DMSO, 5% |
Culture Conditions |
Temperature: 37°C |
STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,12 D16S539: 9,13 D5S818: 11,12 D7S820: 11,12 THO1: 9,10 TPOX: 8 vWA: 18 |
Isoenzymes |
G6PD, B |
Name of Depositor |
GE Foley |
Deposited As |
Homo sapiens |
References |
Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967.
Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381
Santra M, et al. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. J. Clin. Invest. 100: 149-157, 1997. PubMed: 9202067
Qian JX, et al. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 90: 3106-3117, 1997. PubMed: 9376592
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