產(chǎn)品名稱 |
CDC 1C42H11 |
商品貨號(hào) |
B164220 |
Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
Cell Type |
hybridoma: B lymphocyte |
Product Format |
frozen |
Morphology |
lymphoblast |
Culture Properties |
suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Applications |
The antibody reacts with and neutralizes CDV of the Rockborn, Onderstepoort and Snyder Hill strains. The antibody may be useful as a reagent for in vitro testing of CDV vaccines. |
Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
Genes Expressed |
immunoglobulin; monoclonal antibody; against canine distemper virus (CDV) |
Cellular Products |
immunoglobulin; monoclonal antibody; against canine distemper virus (CDV) |
Comments |
Animals were immunized with canine distemper virus (Rockborn strain). Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with and neutralizes CDV of the Rockborn, Onderstepoort and Snyder Hill strains. The antibody may be useful as a reagent for in vitro testing of CDV vaccines. |
Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
|
Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
Isotype |
IgG1 |
Name of Depositor |
TO Bunn |
Deposited As |
mouse (B cell); mouse (myeloma) |