国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CL2
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
CL2
CL2
規(guī)格:
貨期:
編號:B164265
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 CL2
商品貨號 B164265
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
The antibody cross-reacts with canine L-selectin.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
Cellular Products
immunoglobulin; monoclonal antibody; against human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
Comments
Animals were immunized with human umbilical vein endothelial cells (HUVEC) that had been stimulated with recombinant interleukin-1 (IL-1).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
The antibody cross-reacts with canine L-selectin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 1 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2a; kappa light chain
Name of Depositor C Smith
Deposited As mouse (B cell); mouse (myeloma)
References

Kishimoto TK, et al. Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood 78: 805-811, 1991. PubMed: 1713515

Abbassi O, et al. Canine neutrophil margination mediated by lectin adhesion molecule-1 in vitro. J. Immunol. 147: 2107-2115, 1991. PubMed: 1717549

Abbassi O, et al. E-selectin supports neutrophil rolling in vitro under conditions of flow. J. Clin. Invest. 92: 2719-2730, 1993. PubMed: 7504692

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479