| 產(chǎn)品名稱 |
Clone 15 HL-60 |
| 商品貨號(hào) |
B164270 |
| Organism |
Homo sapiens, human |
| Tissue |
peripheral blood |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute promyelocytic leukemia |
| Age |
36 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. |
| Clinical Data |
36 years Caucasian female |
| Receptor Expression |
complement, expressed Fc, expressed interleukin 5 (IL-5), expressed |
| Genes Expressed |
eosinophil granule proteins: eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN) |
| Cellular Products |
eosinophil granule proteins: eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN) |
| Comments |
This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with butyric acid.
Initially, 80% of the treated cells will differentiate as eosinophils.
However, with continued passage the line will gradually exhibit a modest degree of reversion such that a higher percentage of the cells will exhibit neutrophilic differentiation.
The cells express IL-5 receptors and eosinophil granule proteins, and exhibit chemotaxis to eosinophil specific eosinophilotactins. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 X 105 viable cells/mL and subculture at 1 X 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 13,14 D13S317: 8,11 D16S539: 11 D5S818: 12 D7S820: 11,12 THO1: 7,8 TPOX: 8,11 vWA: 16 |
| Name of Depositor |
S Fischkoff |
| Deposited As |
Homo sapiens |
| Year of Origin |
1984 |
| References |
Fischkoff SA. Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions. Leuk. Res. 12: 679-696, 1988. PubMed: 3184985
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