| 產(chǎn)品名稱 |
clone 9 |
| 商品貨號(hào) |
B164272 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
liver |
| Cell Type |
Epithelial |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
4 weeks |
| Gender |
male |
| Strain |
Sprague-Dawley |
| Applications |
This cell line has been used for studies of in vitro carcinogenesis and is useful in clonal assays for screening sera and other nutritional supplements. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Clone 9 (K-9) is an epithelial cell line isolated in 1968 from normal liver taken from a young male rat. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
ME Kaighn |
| Deposited As |
Rattus sp. |
| Year of Origin |
1968 |
| References |
. Gene expression and carcinogenesis in cultured liver. New York: Academic Press; 1975.
Weinstein IB, et al. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253-263, 1975. PubMed: 162864
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