產(chǎn)品名稱 | COS-7 |
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商品貨號 | B164290 |
Organism | Cercopithecus aethiops |
Tissue | kidney |
Cell Type | SV40 transformed |
Product Format | frozen |
Morphology | fibroblast |
Culture Properties | adherent |
Biosafety Level | 2 [Cells Contain SV-40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Applications | This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. |
Storage Conditions | liquid nitrogen vapor phase |
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Derivation | The line was derived from the CV-1 cell line (ATCC CCL-70 ) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. |
Genes Expressed | T antigen |
Cellular Products | T antigen |
Comments | This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40°C, and supports the replication of pure populations of SV40 mutants with deletions in the early region. The line was derived from the CV-1 cell line (ATCC CCL-70 ) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. |
Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
Name of Depositor | Y Gluzman |
Deposited As | Cercopithecus aethiops |
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梅經(jīng)理 | 17280875617 | 1438578920 |
胡經(jīng)理 | 13345964880 | 2438244627 |
周經(jīng)理 | 17757487661 | 1296385441 |
于經(jīng)理 | 18067160830 | 2088210172 |
沈經(jīng)理 | 19548299266 | 2662369050 |
李經(jīng)理 | 13626845108 | 972239479 |