產(chǎn)品名稱 |
CPAE |
商品貨號(hào) |
B164292 |
Organism |
Bos taurus, cow |
Tissue |
pulmonary artery/endothelium |
Cell Type |
endothelial |
Product Format |
frozen |
Morphology |
endothelial |
Culture Properties |
adherent |
Biosafety Level |
2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease |
normal |
Gender |
female |
Storage Conditions |
liquid nitrogen vapor phase |
Karyotype |
normal female; diploid; stable |
Images |
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Derivation |
CPAE is an endothelial cell line derived from the main stem pulmonary artery of a young cow (Bos taurus). This line was initiated in January, 1979 by P. Del Vecchio from artery lumen scrapings dispersed by enzyme treatment. |
Clinical Data |
female |
Genes Expressed |
positive for angiotensin converting enzyme |
Cellular Products |
positive for angiotensin converting enzyme |
Comments |
Subsequent to the publication of the sixth edition of the ATCC catalog, tests for bovine diarrhea virus (BVD) have indicated that CPAE cells test positive for BVD viral antigen. |
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
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Cryopreservation |
culture medium 95%; DMSO, 5% |
Culture Conditions |
Temperature: 37°C
Atmosphere: 95% air; 5% CO2 |
Name of Depositor |
PJ Del Vecchio |
Deposited As |
Bos taurus |
Year of Origin |
1979 |
References |
Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806
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