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CSMalphabeta6C [CSMab6C]
CSMalphabeta6C [CSMab6C]
規(guī)格:
貨期:
編號:B164295
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CSMalphabeta6C [CSMab6C]
商品貨號 B164295
Organism Mus musculus, mouse
Tissue mammary gland
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Papilloma
Gender female
Strain RIII
Applications
produces chorionic gonadotropin, beta polypeptide
produces glycoprotein hormones, alpha polypeptide chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter.
Clinical Data
female
HeLa Markers N
Genes Expressed
glycoprotein hormones, alpha polypeptide(chorionic gonadotropin, alpha polypeptide, luteinizing hormone alpha) [CGA],chorionic gonadotropin, beta polypeptide, glycoprotein hormones, alpha polypeptide
Comments
This line was derived from the C127I mouse fibroblast cell line by transformation with a bovine papilloma virus vector containing the alpha-hCG gene under control of the SV40 early promoter and beta-hCG under control of the metallothionine promoter.
The cells produce very high levels of alpha and beta hCG.
The line contains the plasmid pRF 398 alpha t2.
The construct contains the entire sequence of beta hCG plus 30 bp 5' untranslated and 60 bp 3' untranslated under the control of the mouse metallothionein promoter.
The construct contains the entire sequence of alpha hCG plus 10 bp 5' untranslated and 220 bp 3' untranslated under the control of the SV40 early promoter.
An EcoRI/BamHI fragment of palphat2 (containing alpha hCG and SV40 sequences) was inserted in the BamHI site of pRF398 (ATCC CRL 8401) (containing beta hCG, MT-1 promoter, and BPV sequences) to create pRF398alphat2.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor Genzyme Corp.
U.S. Patent Number
References

Reddy VB, et al. Heteropolymeric protein. US Patent 4,840,896 dated Jun 20 1989

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479