国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CV-1/EBNA-1
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
CV-1/EBNA-1
CV-1/EBNA-1
規(guī)格:
貨期:
編號:B164303
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 CV-1/EBNA-1
商品貨號 B164303
Organism Cercopithecus aethiops
Tissue kidney
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2  [Cells contain herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 141 days
Gender male
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Clinical Data
male
Genes Expressed
Epstein-Barr nuclear antigen 1 (EBNA-1),Expression of EBNA-1 permits episomal replication of expression vectors such as pDC406 that contain the EBV origin of replication.
Cellular Products
Epstein-Barr nuclear antigen 1 (EBNA-1)
Comments

Expression of EBNA-1 permits episomal replication of expression vectors such as pDC406 that contain the EBV origin of replication. The cells can be used as host for expression of transfected DNA.

The line was derived from CV-1 (ATCC CCL-70) by transfection with a plasmid encoding Epstein Barr Virus nuclear antigen 1 (EBNA-1).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove old medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor Immunex Corporation
Deposited As Cercopithecus aethiops
U.S. Patent Number
References

McMahan CJ, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J. 10: 2821-2832, 1991. PubMed: 1833184

Gearing DP. Receptor for oncostatin M and leukemia inhibitory factor. US Patent 5,262,522 dated Nov 16 1993

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479