| 產(chǎn)品名稱 |
DAKIKI |
| 商品貨號(hào) |
B164326 |
| Organism |
Homo sapiens, human |
| Cell Type |
lymphoblast; Epstein-Barr virus (EBV) transforme |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Clinical Data |
Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV. |
| Genes Expressed |
immunoglobulin; monoclonal antibody |
| Cellular Products |
immunoglobulin; monoclonal antibody |
| Comments |
Surface IgA positive human lymphocytes were selected from peripheral blood and cervical lymph node lymphocytes of an African patient with nasopharyngeal carcinoma and subsequently transformed with EBV. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgA; lambda light chain |
| Name of Depositor |
P Ralph |
| Deposited As |
Homo sapiens |
| References |
Steinitz M, Klein G. EBV-transformation of surface IgA-positive human lymphocytes. J. Immunol. 125: 194-196, 1980. PubMed: 6247394
Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester. Immunol. Lett. 7: 17-23, 1983. PubMed: 6605913
|
| Cross References |
Nucleotide (GenBank) :
U07986
Human immunoglobulin IgH heavy chain Fd fragment mRNA, partial cds.
|
