| 產(chǎn)品名稱(chēng) |
DATK32 |
| 商品貨號(hào) |
B164332 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
The antibody reacts with the mouse lymphocyte mucosal homing receptor. It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation. DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against murine alpha 4, beta 7 integrin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against murine alpha 4, beta 7 integrin |
| Comments |
Animals were immunized with cells of the TK1 murine T cell lymphoma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the mouse lymphocyte mucosal homing receptor. It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation. The antibody inhibits alpha 4, beta 7 mediated adhesion activities including binding to MAdCAM-1, and alpha 4, beta 7 dependent VCAM-1 and fibronectin binding. DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining. |
| Complete Growth Medium |
RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
EC Butcher, DP Andrew |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506
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