| 產(chǎn)品名稱 | Daudi |
|---|---|
| 商品貨號 | B164333 |
| Organism | Homo sapiens, human |
| Tissue | peripheral blood |
| Cell Type | B lymphoblast |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Burkitt's lymphoma |
| Age | 16 years |
| Gender | male |
| Ethnicity | Black |
| Applications | Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis. The cell line is also a suitable transfection host |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | Male human karyotype with stemline number of 46. The karyotype is diploid in 66% of the cells and is stable within the stemline. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation | The Daudi line was derived from a 16-year-old Black male with Burkitt's lymphoma by E. Klein and G. Klein in May, 1967. |
| Clinical Data | 16 years
Black
male |
| Receptor Expression | complement, expressed Fc, expressed |
| Tumorigenic | Yes, in nude mice; forms colonies in agarose |
| Effects | Yes, in agarose Yes, in nude mice |
| Comments | The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin (sIg+). ATCC confirmed this cell line is positive for the presence of EBV viral DNA sequences via PCR. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 x 105 viable cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cell density between 3 x 105 and 2 to 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X,Y CSF1PO: 12 D13S317: 11,12 D16S539: 10,12 D5S818: 8,13 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 15,17 |
| Isoenzymes | G6PD, B |
| Isotype | IgM |
| Name of Depositor | G Klein |
| Deposited As | Homo sapiens |
| Year of Origin | May, 1967 |
| References | Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Klein E, et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339 Huber C, et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908 Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896 Gao Y, et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. PubMed: 8985326 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 Montoya JG, et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337 |
| Cross References | Nucleotide (GenBank) : U07990 Human Burkitt's lymphoma immunoglobulin kappa light chain mRNA, partial cds. Nucleotide (GenBank) : U07987 Human Burkitt's lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
