国产黄片大全在线播放_多毛中国农村熟女吟呻对白_欧洲乱码在线播放_亚洲五月婷婷久久综合色

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > DDT1 MF-2
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
DDT1 MF-2
DDT1 MF-2
規(guī)格:
貨期:
編號:B164340
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DDT1 MF-2
商品貨號 B164340
Organism Mesocricetus auratus, hamster, Syrian golden
Tissue ductus deferens; smooth muscle
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease leiomyosarcoma
Gender male
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Receptor Expression
androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic
Comments
Growth of DDT1 MF-2 cells is stimulated by androgens and inhibited by glucocorticoids.
Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene.
Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
To dislodge adherent cells, remove all but 5 to 10 mL of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor JS Norris
Deposited As Mesocricetus auratus
References

Syms AJ, et al. Proliferation of a highly androgen-sensitive ductus deferens cell line (DDT MF-2) is regulated by glucocorticoids and modulated by growth collagen. In Vitro 19: 929-936, 1983. PubMed: 6662552

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479